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dc.contributor.authorRusso, Ilaria
dc.contributor.authorCarrizzo, Albino
dc.contributor.authorBochicchio, Sabrina
dc.contributor.authorPiazza, Ornella
dc.contributor.authorLamberti, Gaetano
dc.contributor.authorBarba, Anna Angela
dc.contributor.authorVecchione, Carmine
dc.contributor.authorZeppa, Pio
dc.contributor.authorIovino, Paola
dc.contributor.authorBucci, Cristina
dc.contributor.authorSantonicola, Antonella
dc.contributor.authorCiacci, Carolina
dc.date.accessioned2020-05-26T10:45:51Z
dc.date.available2020-05-26T10:45:51Z
dc.date.issued2017
dc.identifier.citationRusso I, Albino Carrizzo A, Bochicchio S, Piazza O, Lamberti G, Barba AA, Vecchione C, Zeppa P, Iovino P, Bucci C, Santonicola A, Ciacci C. SiRNA delivery for control of cyclin d1 and e2f1 expression in crohn’s disease. Translational Medicine @ UniSa 2017, 17(5): 25-33.it_IT
dc.identifier.issn2239-9747it_IT
dc.identifier.urihttp://www.translationalmedicine.unisa.it/indexit_IT
dc.identifier.urihttp://elea.unisa.it:8080/xmlui/handle/10556/4494
dc.identifier.urihttp://dx.doi.org/10.14273/unisa-2692
dc.description.abstractEvidence in inflammatory bowel diseases (IBD) supports a connection between inflammation and cancer due to the alteration of the cell cycle with loss of control at the G1/S checkpoint. In this study, we analyze the expression and modulation of CyD1 and E2F1 in colon explants from Crohn's disease (CD) patients. We used ex vivo culture of colon explants from 4 CD patients and 2 healthy controls, stimulated with lipopolysaccharide from Escherichia Coli (EC-LPS). Commercial siRNAs for CyD1 and E2F1 inhibition were encapsulated in Invivofectamine® and in purposely produced nanoliposomal vectors to silencing CyD1 and E2F1 expression. Western blot analysis was used to investigate the effect of siRNA on CyD1, E2F1 and cyclooxygenase 2 (COX-2) expression. In CD patients colon explants, CyD1 and E2F1 increased after the inflammatory stimulus but siRNA silencing attenuated their expression and controlled the COX-2 expression too. These data represent a prelimiary exploration of in vitro siRNA use.it_IT
dc.format.extentP. 25-33it_IT
dc.language.isoenit_IT
dc.sourceUniSa. Sistema Bibliotecario di Ateneoit_IT
dc.subjectIBD (inflammatory bowel disease)it_IT
dc.subjectCrohn’s diseaseit_IT
dc.subjectCyclin D1it_IT
dc.subjectE2F1it_IT
dc.subjectEC-LPS (Lipopolysaccharide from Escherichia Coli)it_IT
dc.subjectsiRNAit_IT
dc.subjectNanoliposomesit_IT
dc.titleSiRNA delivery for control of cyclin d1 and e2f1 expression in crohn’s diseaseit_IT
dc.typeArticleit_IT
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