Please use this identifier to cite or link to this item:
Title: In silico study of protein-protein interactions
Authors: Vangone, Anna
Guerra, Gaetano
Cavallo, Luigi
Sbardella, Gianluca
Sblattero, Daniele
Oliva Romina
Keywords: Proteina;Docking;Biologia
Issue Date: 30-Jan-2013
Publisher: Universita degli studi di Salerno
Abstract: Protein-protein interactions are at the basis of many of the most important molecular processes in the cell, which explains the constantly growing interest within the scientific community for the structural characterization of protein complexes.1 However, experimental knowledge of the 3D structure of the great majority of such complexes is missing, and this spurred their accurate prediction through molecular docking simulations, one of the major challenges in the field of structural computational biology and bioinformatics.2,3 My PhD work aims to contribute to the field, by providing novel computational instruments and giving useful insight on specific case studies in the field. In particular, in the first part of my PhD thesis, I present novel methods I developed: i) for analysing and comparing the 3D structure of protein complexes, to immediately extract useful information on the interaction based on a contact map visualization (COCOMAPS4 web tool, Chapter 2), and ii) for analysing a set of multiple docking solutions, to single out the key inter-residue contacts and to distinguish native-like solutions from the incorrect ones (CONS-COCOMAPS5 web tool and CONS-RANK program, Chapter 3 and 4, respectively). In the second part of the thesis, these methods have been applied, in combination with classical state-of-art computational biology techniques, to predict and analyse the binding mode in real biological systems, related to particular diseases. This part of the work has been afforded in collaboration with experimental groups, to take advantage of specific biological information on the systems under study. In particular, the interaction between proteins involved in the autoimmune response in celiac disease6,7 (Chapters 5 and 6) has been studied in collaboration with the group directed by Prof. Sblattero, University of Piemonte Orientale (Italy) and the group directed by Prof. Esposito, University of Salerno (Italy). In addition, recognition properties of 3 the FXa enzymatic system8 has been studied through dynamic characterization of a FXa pathogenic mutant that causes problems in the blood coagulation cascade (Chapter 7). This study has been performed in collaboration with the group directed by Prof. De Cristofaro, Catholic University School of Medicine, Rome (Italy) and the group directed by Prof. Peyvandi, Ospedale Maggiore Policlinico and Università degli Studi di Milano (Italy)... [edited by author]
Description: 2011 - 2012
Appears in Collections:Scienza e tecnologie per l’industria chimica, farmaceutica e alimentare

Files in This Item:
File Description SizeFormat 
tesi A. Vangone.pdftesi di dottorato27,65 MBAdobe PDFView/Open
Abstract in inglese A. Vangone.pdfabstract in inglese a cura dell'autore231,85 kBAdobe PDFView/Open
Abstract in italiano A. vangone.pdfabstract in italiano a cura dell'autore221,04 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.